chromograinin a Search Results


gene exp chga mm00514341 m1  (Thermo Fisher)
96
Thermo Fisher gene exp chga mm00514341 m1
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Gene Exp Chga Mm00514341 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
gene exp chga mm00514341 m1 - by Bioz Stars, 2026-02
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rabbit polyclonal anti cga  (Novus Biologicals)
94
Novus Biologicals rabbit polyclonal anti cga
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Rabbit Polyclonal Anti Cga, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
rabbit polyclonal anti cga - by Bioz Stars, 2026-02
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primary antibodies against human chromogranin  (Agilent technologies)
90
Agilent technologies primary antibodies against human chromogranin
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Primary Antibodies Against Human Chromogranin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against human chromogranin/product/Agilent technologies
Average 90 stars, based on 1 article reviews
primary antibodies against human chromogranin - by Bioz Stars, 2026-02
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chromogranin thermo scientific 1:800  (Thermo Fisher)
90
Thermo Fisher chromogranin thermo scientific 1:800
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Chromogranin Thermo Scientific 1:800, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
chromogranin thermo scientific 1:800 - by Bioz Stars, 2026-02
90/100 stars
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anti-chromogranin daka3  (Agilent technologies)
90
Agilent technologies anti-chromogranin daka3
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Anti Chromogranin Daka3, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-chromogranin daka3/product/Agilent technologies
Average 90 stars, based on 1 article reviews
anti-chromogranin daka3 - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti secretogranin ii  (Thermo Fisher)
86
Thermo Fisher rabbit anti secretogranin ii
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Rabbit Anti Secretogranin Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
rabbit anti secretogranin ii - by Bioz Stars, 2026-02
86/100 stars
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chr a chromogranin a  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology chr a chromogranin a
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Chr A Chromogranin A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
chr a chromogranin a - by Bioz Stars, 2026-02
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chromogranin  (Boehringer Mannheim)
90
Boehringer Mannheim chromogranin
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Chromogranin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
chromogranin - by Bioz Stars, 2026-02
90/100 stars
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primary antibodies against chromogranin a (cg-a)  (OriGene)
90
OriGene primary antibodies against chromogranin a (cg-a)
a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, <t>CHGA,</t> and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).
Primary Antibodies Against Chromogranin A (Cg A), supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against chromogranin a (cg-a)/product/OriGene
Average 90 stars, based on 1 article reviews
primary antibodies against chromogranin a (cg-a) - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti chgb antibody  (Proteintech)
93
Proteintech rabbit anti chgb antibody
Changes to the cellular composition of the crypt. (A, C, G, and K) Temporal measurements of CCH (A), the <t>crypt</t> <t>PCNA</t> + zone (C), goblet cell density (G), and <t>CHGB-positive</t> cell density (K). Each point represents the mean for an individual mouse. *, P < 0.0.5; ****, P < 0.0001 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected {UI} mice]). (B and L) Representative distal colonic sections showing temporal changes in the crypt PCNA + zone (B) and CHGB-positive cell density (L). Bars = 50 μm (B) or 100 μm (L). (D and F) Average Log 2 FC values of selected significantly changed (FDR of <0.05 by one-way ANOVA) proteins involved in cell proliferation and cIEC metabolic changes (D) and cIEC markers (F). DSC, deep secretory cell. In panel F, “−ve” indicates that proteins are negative regulators of terminal differentiation into the specified cell type. (E, I, and J) qRT-PCR of the indicated mRNAs isolated from cIECs. Each point represents an individual mouse. *, P < 0.05; **, P < 0.01 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected mice]). For panels G and J, square data points were identified as outliers and not included in the statistical analysis. (H) z scores of selected protein abundances across the entire C. rodentium infection time course (4 to 48 DPI). Means ± standard errors of the means (SEM) are shown.
Rabbit Anti Chgb Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti chgb antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti chgb antibody - by Bioz Stars, 2026-02
93/100 stars
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anti chromogranin a  (Proteintech)
95
Proteintech anti chromogranin a
Changes to the cellular composition of the crypt. (A, C, G, and K) Temporal measurements of CCH (A), the <t>crypt</t> <t>PCNA</t> + zone (C), goblet cell density (G), and <t>CHGB-positive</t> cell density (K). Each point represents the mean for an individual mouse. *, P < 0.0.5; ****, P < 0.0001 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected {UI} mice]). (B and L) Representative distal colonic sections showing temporal changes in the crypt PCNA + zone (B) and CHGB-positive cell density (L). Bars = 50 μm (B) or 100 μm (L). (D and F) Average Log 2 FC values of selected significantly changed (FDR of <0.05 by one-way ANOVA) proteins involved in cell proliferation and cIEC metabolic changes (D) and cIEC markers (F). DSC, deep secretory cell. In panel F, “−ve” indicates that proteins are negative regulators of terminal differentiation into the specified cell type. (E, I, and J) qRT-PCR of the indicated mRNAs isolated from cIECs. Each point represents an individual mouse. *, P < 0.05; **, P < 0.01 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected mice]). For panels G and J, square data points were identified as outliers and not included in the statistical analysis. (H) z scores of selected protein abundances across the entire C. rodentium infection time course (4 to 48 DPI). Means ± standard errors of the means (SEM) are shown.
Anti Chromogranin A, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti chromogranin a/product/Proteintech
Average 95 stars, based on 1 article reviews
anti chromogranin a - by Bioz Stars, 2026-02
95/100 stars
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chromogranin a(lk2h10)  (Biotium)
99
Biotium chromogranin a(lk2h10)
Changes to the cellular composition of the crypt. (A, C, G, and K) Temporal measurements of CCH (A), the <t>crypt</t> <t>PCNA</t> + zone (C), goblet cell density (G), and <t>CHGB-positive</t> cell density (K). Each point represents the mean for an individual mouse. *, P < 0.0.5; ****, P < 0.0001 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected {UI} mice]). (B and L) Representative distal colonic sections showing temporal changes in the crypt PCNA + zone (B) and CHGB-positive cell density (L). Bars = 50 μm (B) or 100 μm (L). (D and F) Average Log 2 FC values of selected significantly changed (FDR of <0.05 by one-way ANOVA) proteins involved in cell proliferation and cIEC metabolic changes (D) and cIEC markers (F). DSC, deep secretory cell. In panel F, “−ve” indicates that proteins are negative regulators of terminal differentiation into the specified cell type. (E, I, and J) qRT-PCR of the indicated mRNAs isolated from cIECs. Each point represents an individual mouse. *, P < 0.05; **, P < 0.01 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected mice]). For panels G and J, square data points were identified as outliers and not included in the statistical analysis. (H) z scores of selected protein abundances across the entire C. rodentium infection time course (4 to 48 DPI). Means ± standard errors of the means (SEM) are shown.
Chromogranin A(Lk2h10), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromogranin a(lk2h10)/product/Biotium
Average 99 stars, based on 1 article reviews
chromogranin a(lk2h10) - by Bioz Stars, 2026-02
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Image Search Results


a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, CHGA, and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).

Journal: Nature Communications

Article Title: Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

doi: 10.1038/s41467-022-29279-4

Figure Lengend Snippet: a Brightfield images of gastric organoids grown in SEM hydrogels and Matrigel (MAT) at day 5 (scale bar = 200 µm, independent experiments = 3). b Quantification of gastric organoid formation efficiency in SEM hydrogels compared to in MAT ( N = 6, independent experiments = 3). c qPCR analysis to compare mRNA expression in gastric organoids grown in each hydrogel (SEM 7 mg ml −1 versus MAT, * p = 0.0174 for Pgc , ** p = 0.0051 for Atp4a , *** p < 0.0001 for Atp4b ; SEM 5 mg ml −1 versus MAT, ** p = 0.0029; N = 4, independent experiments = 3). d Comparison of mRNA expression in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT ( N = 4, independent experiments = 3). e Immunofluorescent staining for stemness markers (SOX9 and KI67), differentiation markers (MUC5AC, CHGA, and HK), a tight junction marker (ZO1), and a cell–cell adhesion/interaction marker (ECAD) in gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). f Fluorescent staining with acridine orange for gastric organoids grown in 5 mg ml −1 SEM hydrogel and MAT (scale bars = 100 µm), and quantification of fluorescence (600–650 nm)/fluorescence (500–550 nm) from organoids in each hydrogel ( N = 12 for SEM and N = 14 for MAT, independent experiments = 2). The color scale indicates the relative number of pixels displayed in the area. g Brightfield images of intestinal organoids grown in IEM hydrogels and MAT at day 6 (scale bar = 200 µm, independent experiments = 3). h Quantification of intestinal organoid formation efficiency in IEM hydrogels compared to in MAT ( N = 4, independent experiments = 3). i qPCR analysis to compare mRNA expression of intestinal organoids within each hydrogel (IEM 2 mg ml −1 versus MAT, *** p < 0.0001 for Lgr5 , *** p = 0.0009 for Muc2 ; IEM 3 mg ml −1 versus MAT, *** p = 0.0003 for Lgr5 , ** p = 0.0032 for Muc2 ; IEM 4 mg ml −1 versus MAT, * p = 0.0455 for Muc2 ; N = 4, independent experiments = 3). j Comparison of mRNA expression of intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (IEM versus MAT, *** p < 0.0001 for Lgr5 , *** p < 0.0001 for Axin2 , ** p = 0.0035 for Muc2 ; N = 4, independent experiments = 3). k Immunofluorescent staining for a stemness marker, differentiation markers (MUC2, LYZ, CHGA, and VILLIN), a tight junction marker, and a cell–cell adhesion/interaction marker in intestinal organoids grown in 2 mg ml −1 IEM hydrogel and MAT (scale bars = 50 µm, independent experiments = 3). l Brightfield images of intestinal organoids grown in each hydrogel after forskolin treatment (scale bar = 100 µm), and m the area of forskolin-treated organoids normalized to the organoid area prior to forskolin treatment in each group ( N = 4, independent experiments = 3). The data in b – d , f , h – j , and m are presented as mean ± S.D. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test ( c , i ) and unpaired, two-sided student’s t -test ( d , j ).

Article Snippet: The following TaqMan gene expression assay kits were used for qPCR: Lgr5 (Mm00438890_m1), Pgc (Mm00482488_m1), Atp4a (Mm00444417_m1), Atp4b (Mm00437657_m1), Axin2 (Mm00443610_m1), Muc6 (Mm00725165_m1), Gif (Mm00433596_m1), Pga5 (Mm01208256_m1), Muc2 (Mm00458299_m1), Olfm4 (Mm01320260_m1), Lyz1 (Mm00657323_m1), Chga (Mm00514341_m1), Vil1 (Mm00494146_m1), and Casp3 (Mm01195085_m1).

Techniques: Expressing, Comparison, Staining, Marker, Fluorescence

Changes to the cellular composition of the crypt. (A, C, G, and K) Temporal measurements of CCH (A), the crypt PCNA + zone (C), goblet cell density (G), and CHGB-positive cell density (K). Each point represents the mean for an individual mouse. *, P < 0.0.5; ****, P < 0.0001 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected {UI} mice]). (B and L) Representative distal colonic sections showing temporal changes in the crypt PCNA + zone (B) and CHGB-positive cell density (L). Bars = 50 μm (B) or 100 μm (L). (D and F) Average Log 2 FC values of selected significantly changed (FDR of <0.05 by one-way ANOVA) proteins involved in cell proliferation and cIEC metabolic changes (D) and cIEC markers (F). DSC, deep secretory cell. In panel F, “−ve” indicates that proteins are negative regulators of terminal differentiation into the specified cell type. (E, I, and J) qRT-PCR of the indicated mRNAs isolated from cIECs. Each point represents an individual mouse. *, P < 0.05; **, P < 0.01 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected mice]). For panels G and J, square data points were identified as outliers and not included in the statistical analysis. (H) z scores of selected protein abundances across the entire C. rodentium infection time course (4 to 48 DPI). Means ± standard errors of the means (SEM) are shown.

Journal: mBio

Article Title: Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium

doi: 10.1128/mbio.03233-21

Figure Lengend Snippet: Changes to the cellular composition of the crypt. (A, C, G, and K) Temporal measurements of CCH (A), the crypt PCNA + zone (C), goblet cell density (G), and CHGB-positive cell density (K). Each point represents the mean for an individual mouse. *, P < 0.0.5; ****, P < 0.0001 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected {UI} mice]). (B and L) Representative distal colonic sections showing temporal changes in the crypt PCNA + zone (B) and CHGB-positive cell density (L). Bars = 50 μm (B) or 100 μm (L). (D and F) Average Log 2 FC values of selected significantly changed (FDR of <0.05 by one-way ANOVA) proteins involved in cell proliferation and cIEC metabolic changes (D) and cIEC markers (F). DSC, deep secretory cell. In panel F, “−ve” indicates that proteins are negative regulators of terminal differentiation into the specified cell type. (E, I, and J) qRT-PCR of the indicated mRNAs isolated from cIECs. Each point represents an individual mouse. *, P < 0.05; **, P < 0.01 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected mice]). For panels G and J, square data points were identified as outliers and not included in the statistical analysis. (H) z scores of selected protein abundances across the entire C. rodentium infection time course (4 to 48 DPI). Means ± standard errors of the means (SEM) are shown.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti‐ C. rodentium antibody (1:50) (Statens Serum Institute, Copenhagen, Denmark), mouse anti-PCNA antibody (1:500) (catalog number ab29; Abcam), and rabbit anti-CHGB antibody (1:50) (catalog number 14968-1-AP; Proteintech).

Techniques: Control, Quantitative RT-PCR, Isolation, Infection